Archive for the ‘CELL’ Tag

VIRUSES   Leave a comment

DEFINITION:1 orig., venom, as of a snake 2 a) any of a kingdom (Virus) of prokaryotes, usually ultra microscopic, that consist of nucleic acid, either RNA or DNA, within a case of protein: they infect animals, plants, and bacteria and can reproduce only within living cells so that they are considered as being either living organisms or inert chemicals b) a disease caused by a virus 3 anything that corrupts or poisons the mind or character; evil or harmful influence 4 an unauthorized, disruptive set of instructions placed in a computer program, that leaves copies of itself in other programs and disks.

1981: US AIDS diagnosed. A new fatal, infectious disease was diagnosed in 1981. Called Acquired Immunodeficiency Syndrome (AIDS), it began appearing in major cities among homosexual men and intravenous drug users. Other high-risk groups were haemophiliacs and other recipients of blood or blood products, babies born of AIDS-infected women, bisexual men, and prostitutes and their customers. AIDS was soon recognized as a worldwide health emergency: a fatal disease with no known cure that quickly became an epidemic. It was especially widespread in Africa, the apparent land of its origin.

By 1983 the virus that causes the disease had been isolated. Some medicines, notably AZT (azidothymidine), slowed the disease’s progress for a few months or more; but the spread of AIDS continued relentlessly, with more than 3,000 new cases being reported each month by 1991.

The federal government had committed more than 1.6 billion dollars to research, while the homosexual community and other special interest groups sought more federal funding and greater assistance from the health insurance industry. Educational programs on safe sexual practices, such as the use of condoms, seemed the best means of slowing the epidemic. Meanwhile, more than 70,000 persons in the United States had died from AIDS by the end of the decade.

1981: WORLD AIDS identified. A strange, new, and deadly disease made its appearance in 1980. Physicians in such large cities as Los Angeles, New York, and San Francisco noticed that homosexual men were dying from rare lung infections or from a cancer known as Kaposi’s sarcoma. By 1981 the disease was identified and given a name: AIDS, or acquired immunodeficiency syndrome.

The virus that causes AIDS, human immunodeficiency virus (HIV), was identified by Dr. Luc Montagnier of the Pasteur Institute in Paris in research done during the years 1981-84. The results of Dr. Montagnier’s studies were released in 1984. Since its discovery, AIDS has become one of the world’s major health problems. Within certain populations it has become an epidemic: male homosexuals, haemophiliacs, and intravenous drug users in the United States, for example, and heterosexual men and women in Sub-Saharan Africa. Many people were infected through blood transfusions before HIV screening was introduced. An individual infected with the virus may not show the symptoms of AIDS for several years, but the condition is eventually fatal.

The search for a successful vaccine was pursued in laboratories around the world, with no success by the early 1990s. Meanwhile, the disease continued to spread to different parts of the world. Already rife in the United States, Europe, and sub-Saharan Africa by the mid-1980s, it quickly spread to Central and East Asia. The disease also began to spread to larger portions of the heterosexual community throughout the world.

The composition of a virus is relatively simple, and its size is extremely small. It cannot even properly be called an organism because it is unable to carry on life processes outside a living cell of an animal, plant, or bacteria. Yet its method of entering and “enslaving” a living cell is so ingenious that the virus is humankind’s deadliest enemy and resists the most advanced efforts of modern science to eliminate it.

Millions of people throughout the world suffer each year from viral diseases such as polio, measles, chicken pox, mumps, acquired immunodeficiency syndrome (AIDS), and the common cold. Viruses also produce such illnesses as foot-and-mouth disease in livestock, distemper in dogs, panleukopenia in cats, and hog cholera. The viruses that infect bacteria are called bacteriophages.

Structure and Composition

Nucleic acid, any of substances comprising genetic material of living cells; divided into two classes: RNA (ribonucleic acid) and DNA (deoxyribonucleic acid); directs protein synthesis and is vehicle for transmission of genetic information from parent to offspring.

Viruses are exceedingly small; they range in size from about 0.02 to 0.25 micron in diameter (1 micron = 0.000039 inch). By contrast, the smallest bacteria are about 0.4 micron in size. As observed with an electron microscope, some viruses are rod-shaped, others are roughly spherical, and still others have complex shapes consisting of a multi sided “head” and a cylindrical “tail.” A virus consists of a core of nucleic acid surrounded by a protein coat called a capsid; some viruses also have an outer envelope composed of fatty materials and proteins. The nucleic-acid core is the essential part of the virus it carries the virus’s genes. The core consists of either deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), substances that are essential to the transmission of hereditary information. The protein capsid protects the nucleic acid and may contain molecules that enable the virus to enter the host cell that is, the living cell infected by the virus.

Cycle and Patterns of Infection

Outside of a living cell, a virus is a dormant particle. It exhibits none of the characteristics generally associated with life namely, reproduction and metabolic processes such as growth and assimilation of food. Unlike bacteria and other micro-organisms, viruses remain dormant in body fluids. Thus, great numbers of viruses may be present in a body and yet not produce a disease because they have not invaded the body’s cells. Once inside a host cell, however, the virus becomes an active entity capable of taking over the infected cell’s metabolic machinery. The cellular metabolism becomes so altered that it helps to produce thousands of new viruses.

The virus’s developmental cycle begins when it succeeds in introducing its nucleic acid, and in some cases its protein coat, into a host cell. Bacteriophages attach to the surface of the bacterium and then penetrate the rigid cell wall, transmitting the viral nucleic acid into the host. Animal viruses enter host cells by a process called endocytosis. Plant viruses enter through wounds in the cell’s outer coverings through abrasions made by wind, for example, or through punctures made by insects.

Virion, an entire virus particle the extracellular infective form of a virus consisting of an outer protein shell (capsid) and an inner core of nucleic acid (either ribonucleic or deoxyribonucleic acid); in some, the capsid further enveloped by a fatty membrane.

Once inside the host cell, the virus’s genes usually direct the cell’s production of new viral protein and nucleic acid. These components are then assembled into new, complete, infective virus particles called virions, which are then discharged from the host cell to infect other cells.

In the case of bacteriophages, the new virions are usually released by bursting the host cell a process called lysing, which kills the cell. Sometimes, however, bacteriophages form a stable association with the host cell. The virus’s genes are incorporated into the host cell’s genes, replicate as the cell’s genes replicate, and when the cell divides, the viral genes are passed on to the two new cells.

In such cases no virions are produced, and the infecting virus seems to disappear. Its genes, however, are being passed on to each new generation of cells that stem from the original host cell. These cells remain healthy and continue to grow unless, as happens occasionally, something triggers the latent viral genes to become active. When this happens, the normal cycle of viral infection results: the viral genes direct viral replication, the host cell bursts, and the new virions are released. This pattern of infection is called lysogeny.

Closely related to lysogeny is the process known as transduction, whereby a virus carries bacterial genes from one host to another. This transduction process occurs when genes from the original host become incorporated into a virion that subsequently infects another bacterium.

Viral infections of plant and animal cells resemble those of bacterial cells in many ways. The release of new virions from plant and animal cells does not, however, always involve the bursting of the host cell as it does in bacteria. Particularly among animal viruses, the new virions may be released by budding off from the cell membrane, a process that does not necessarily kill the host cell.

In general, a viral infection produces one of four effects in a plant or animal cell: in apparent effect, in which the virus remains dormant in the host cell; cytopathic effect, in which the cell dies; hyperplastic effect, in which the cell is stimulated to divide before its death; and cell transformation, in which the cell is stimulated to divide, take on abnormal growth patterns, and become cancerous.

Cold sore (or fever blister, or Herpes simplex), a virus infection of the borders of the mouth, lips and nose, or genitals; marked by watery blisters; may be due to illness, emotional upset, or other stress.

Viral infections in animals can be localized or can spread to various parts of the body. Some animal viruses produce latent infections: the virus remains dormant much of the time but becomes active periodically. This is the case with the herpes simplex viruses that cause cold sores.

Natural Defences, Immunization, Treatment

Fever, a condition in which the body temperature rises above normal.

Animals have a number of natural defences that may be triggered by a viral infection. Fever is a general response; many viruses are inactivated at temperatures just slightly above the host’s normal body temperature. Another general response of infected animal cells is the secretion of a protein called interferon. Interferon inhibits the reproduction of viruses in non infected cells.

Fever and interferon production are general responses to infection by any virus. In addition, humans and other vertebrates can mount an immunological attack against a specific virus. The immune system produces antibodies and sensitized cells that are tailor-made to neutralize the infecting virus. These immune defenders circulate through the body long after the virus has been neutralized, thereby providing long-term protection against reinfection by that virus.

Such long-term immunity is the basis for active immunization against viral diseases. A weakened or inactivated strain of an infectious virus is introduced into the body. This virus does not provoke an active disease state, but it does stimulate the production of immune cells and antibodies, which then protect against subsequent infection by the virulent form of the virus.

Active immunizations are routine for such viral diseases as measles, mumps, poliomyelitis, and rubella. In contrast, passive immunization is the injection of antibodies from the serum of an individual who has already been exposed to the virus. Passive immunization is used to give short-term protection to individuals who have been exposed to such viral diseases as measles and hepatitis. It is useful only if provided soon after exposure, before the virus has become widely disseminated in the body.

The treatment of an established viral infection usually is restricted to relieving specific symptoms. There are few drugs that can be used to combat a virus directly. The reason for this is that viruses use the machinery of living cells for reproduction. Consequently, drugs that inhibit viral development also inhibit the functions of the host cell. Nonetheless, a small number of antiviral drugs are available for specific infections.

The most successful controls over viral diseases are epidemiological. For example, large-scale active immunization programs can break the chain of transmission of a viral disease. Worldwide immunization is credited with the eradication of smallpox, once one of the most feared viral diseases. Because many viruses are carried from host to host by insects or contaminated food, insect control and hygienic food handling can help eliminate a virus from specific populations.

History of Virus Research

Historic descriptions of viral diseases date back as far as the 10th century BC. The concept of the virus, however, was not established until the last decade of the 19th century, when several researchers obtained evidence that agents far smaller than bacteria were capable of causing infectious diseases.

Mosaic disease, highly infectious virus disease affecting many plants including cucumber, potato, tomato, bean, and turnip; dwarfs growth and mottles leaves.

The existence of viruses was finally proved when bacteriophages were discovered by independent researchers in 1915 and 1917. The question of whether viruses are actually micro-organisms (similar to very tiny bacteria) was resolved in 1935, when the virus responsible for causing mosaic disease in tobacco was isolated and crystallized; the fact that it could be crystallized proved that the virus was not a cellular organism.

Bacteriophages are a valuable research tool for molecular biologists. Studies of bacteriophages have helped to illuminate such basic biological processes as genetic recombination, nucleic-acid replication, and protein synthesis.

GENETICS (Part 3 of 3)   Leave a comment

Sex Linkage

Linked genes occur on the sex chromosomes as well as on the non sex chromosomes, or autosomes. In humans, a woman carries two X chromosomes and 44 autosomes in each body cell and one X chromosome and 22 autosomes in each egg. A man carries one X and one Y chromosome and 44 autosomes in each body cell and either an X or a Y chromosome and 22 autosomes in each sperm cell.

Only sons inherit traits carried by genes located on the Y chromosome, because a boy (XY) develops whenever a Y sperm fertilizes an egg. Traits carried on genes located on an X chromosome of the father are transmitted only to daughters (XX).

GENES AND THE GENETIC CODE

Genes, the arbiters of body form and organ function, work with precision. They transmit to each cell a genetic code that determines the cell’s purpose.

Nucleic Acids The Key to Heredity

Nucleic acid, any of substances comprising genetic material of living cells; divided into two classes: RNA (ribonucleic acid) and DNA (deoxyribonucleic acid); directs protein synthesis and is vehicle for transmission of genetic information from parent to offspring.

The structure of DNA makes gene transmission possible. Since genes are segments of DNA, DNA must be able to make exact copies of itself to enable the next generation of cells to receive the same genes.

Adenine, a purine base that codes hereditary information in the genetic code in DNA and RNA.

Cytosine, pyrimidine base that codes genetic information in DNA or RNA.

The DNA molecule looks like a twisted ladder. Each “side” is a chain of alternating phosphate and deoxyribose sugar molecules. The “steps” are formed by bonded pairs of purine-pyrimidine bases. DNA contains four such bases the purines adenine (A) and guanine (G) and the pyrimidines cytosine (C) and thymine (T).

The RNA molecule, markedly similar to DNA, usually consists of a single chain. The RNA chain contains ribose sugars instead of deoxyribose. In RNA, the pyrimidine uracil (U) replaces the thymine of DNA.

DNA and RNA are made up of basic units called nucleotides. In DNA, each of these is composed of a phosphate, a deoxyribose sugar, and either A, T, G, or C. RNA nucleotides consist of a phosphate, a ribose sugar, and either A, U, G, or C.

Nucleotide chains in DNA wind around one another to form a complete twist, or gyre, every ten nucleotides along the molecule. The two chains are held fast by hydrogen bonds linking A to T and C to G A always pairs with T (or with U in RNA); C always pairs with G. Sequences of the paired bases are the foundation of the genetic code. Thus, a portion of a double-stranded DNA molecule might read: A-T C-G G-C T-A G-C C-G A-T. When “unzipped,” the left strand would read: ACGTGCA; the right strand: TGCACGT.

DNA is the “master molecule” of the cell. It directs the synthesis of RNA. When RNA is being transcribed, or copied, from an unzipped segment of DNA, RNA nucleotides temporarily pair their bases with those of the DNA strand. In the preceding example, the left hand portion of DNA would transcribe a strand of RNA with the base sequence: UGCACGU.

Genes and Protein Synthesis

A genetic code guides the assembly of proteins. The code ensures that each protein is built from the proper sequence of amino acids.

Genes transmit their protein-building instructions by transcribing a special type of RNA called messenger RNA (mRNA). This leaves the cell nucleus and moves to structures in the cytoplasm called ribosomes, where protein synthesis takes place.

Cell biologists believe that DNA also builds a type of RNA called transfer RNA (tRNA), which floats freely through the cell cytoplasm. Each tRNA molecule links with a specific amino acid. When needed for protein synthesis, the amino acids are borne by tRNA to a ribosome.

For years biologists wondered how amino acids were guided to fit together in the exact sequences needed to produce the thousands of kinds of proteins required to sustain life. The answer seems to lie in the way the four genetic “code letters” A, T, C, and G are arranged along the DNA molecule.

The Genetic Code

Experimental evidence indicates that the genetic code is a “triplet” code; that is, each series of three nucleotides along the DNA molecule orders where a particular amino acid should be placed in a growing protein molecule. Three-nucleotide units on an mRNA strand for example UUU, UUG, and GUU are called codons. The codons, transcribed from DNA, are strung out in a sequence to form mRNA.

According to the triplet theory, tRNA contains anti codons, nucleotide triplets that pair their bases with mRNA codons. Thus, AAA is the anti codon for UUU. When a codon specifies a particular amino acid during protein synthesis, the tRNA molecule with the anti codon delivers the needed amino acid to the bonding site on the ribosome.

The genetic code consists of 64 codons. However, since these codons order only some 20 amino acids, most, if not all, of the amino acids can be ordered by more than one of them. For example, the mRNA codons UGU and UGC both order cysteine. Because mRNA is a reverse copy of DNA the genetic code for cysteine is ACA or ACG. Some codons may act only to signal a halt to protein synthesis.

To illustrate the operation of the genetic code, assume that one protein is responsible for the development of brown hair and that this protein is composed of three amino acid molecules arranged in linear sequence for example, cysteine-cysteine-cysteine. (This is a much simplified example, since proteins actually incorporate from 100 to 300 amino acid molecules.) The gene (DNA segment) specifying formation of this protein reads: ACAACAACA. It produces the mRNA segment UGUUGUUGU. This segment then drifts to a ribosome. Three tRNA molecules, each with the cysteine-bearing anti codon ACA, line up in order on the ribosome and deposit their cysteine to make the brown-hair protein.

Since code transmission from DNA to mRNA is extremely precise, any error in the code affects protein synthesis. If the error is serious enough, it eventually affects some body trait or feature.

Mutations

Down’s syndrome (or mongolism), a congenital condition with moderate to severe mental retardation; characteristic features include: broad flat faces, slanted eyes, small ears and noses; heart defects and other abnormalities.

Certain chemicals and types of radiation can cause mutations changes in the structure of genes or chromosomes. The simplest type of mutation is a change in the DNA or RNA nucleotide sequence. Mutations may also involve the number of chromosomes or the gain, loss, or rearrangement of chromosome segments. If a mutation occurs in parental sex cells, the change is passed on to the offspring. In humans, an extra chromosome in body cells (47 instead of 46) has been implicated in Down’s syndrome, a serious mental abnormality.

Most mutations are considered harmful and are, therefore, eventually eliminated. Some, however, enable an organism to adapt to a changing environment. Biologists believe that mutations have caused the many genetic changes involved in the evolution of species.

Assisted by Val W. Woodward

Genetic Terms

allele. One of the members of a gene pair, each of which is found on chromosomes; the pair of alleles determines a specific trait.

chromosome. A structure in the cell nucleus containing genes.

dominance. The expression of one member of an allelic pair at the expense of the other in the phenotypes of heterozygotes.

gene. One of the chromosomal units that transmit specific hereditary traits; a segment of the self-reproducing molecule, deoxyribonucleic acid.

genotype. The genetic make-up of an organism, which may include genes for the traits that do not show up in the phenotype.

heterozygous. Containing dissimilar alleles.

homozygous. Containing a pair of identical alleles.

phenotype. The visible characteristics of an organism (for example, height and colouration).

recessiveness. The masking of one member of an allelic pair by the other in the phenotypes of heterozygotes.

Posted 2012/04/19 by Stelios in Education

Tagged with , , ,